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Image Search Results
Journal: Pharmaceuticals
Article Title: Cytochalasin B Influences Cytoskeletal Organization and Osteogenic Potential of Human Wharton’s Jelly Mesenchymal Stem Cells
doi: 10.3390/ph16020289
Figure Lengend Snippet: Immunofluorescence of human Wharton’s jelly mesenchymal stem cell (hWJ-MSC) cytoskeletal markers after 24 h of Cytochalasin B (CB) treatment. hWJ-MSCs were immunostained with Phalloidin (grey signal, specific for F-Actin), anti-vimentin (green signal), or anti-vinculin (red signal) after 24 h of dimethyl sulfoxide (DMSO) 0.05% (CB vehicle) or CB (0.1, 1, or 3 μM) exposure. Untreated cells were used as CTR. NucBlue ® Fixed Cell ReadyProbes ® Reagent (DAPI) was used to counter-stain nuclei (blue signal). Images were acquired using a Nikon inverted microscope Eclipse Ti2-E and a digital sight camera DS-Qi2, through the imaging software NIS-Elements. Scale bars: 50 μm.
Article Snippet: The detection and acquisition of images was performed using Nikon
Techniques: Immunofluorescence, Staining, Inverted Microscopy, Imaging, Software
Journal: Pharmaceuticals
Article Title: Cytochalasin B Influences Cytoskeletal Organization and Osteogenic Potential of Human Wharton’s Jelly Mesenchymal Stem Cells
doi: 10.3390/ph16020289
Figure Lengend Snippet: Immunofluorescence analysis in human Wharton’s jelly mesenchymal stem cells (hWJ-MSCs) of cytoskeletal and osteogenic markers at 7 days from the beginning of the osteogenic program. hWJ-MSCs were cultured in osteogenic medium with dimethyl sulfoxide (DMSO) 0.05% (CB vehicle) or CB 1 μM for the entire experimental time (continuous CB). hWJ-MSCs were also cultured in osteogenic medium (OM) in presence of CB 1 μM for only 24 h, and then removed, and cells were cultured in OM up to the end of the experiment (wo = CB wash-out). hWJ-MSCs were immunostained with Phalloidin (grey signal, specific for F-Actin) or anti-osteopontin (green signal) at 7 days from the beginning of the osteogenic program. NucBlue ® Fixed Cell ReadyProbes ® Reagent (DAPI) was used to counter-stain nuclei (blue signal). Images were acquired using a Nikon inverted microscope Eclipse Ti2-E and a digital sight camera DS-Qi2, through the imaging software NIS-Elements. Scale bars: 100 μm.
Article Snippet: The detection and acquisition of images was performed using Nikon
Techniques: Immunofluorescence, Cell Culture, Staining, Inverted Microscopy, Imaging, Software